Comparative Evaluation of the Anti-Angiogenic Properties of Vitamin B Complex and Ascorbic Acid using Duck Embryo Chorioallantoic Membrane (CAM) Assay

AILYN B. ANGLO-OJEDA1, APRIL B. FLORES2, AVRIL LEY ANN V. RECTO3
Biological Sciences Department, Graduate School-College of Science and Computer Studies,
De La Salle University – Dasmariñas, Cavite Philippines1-3

DOI: https://doi.org/10.54476/8028643

 

ABSTRACT

With tens of millions of people diagnosed with cancer each year, and with the increasing attention on the use of vitamins as an important part of human health, investigating the effect of essential vitamins on angiogenesis which is a critical step in cancer development plays a significant role in fighting the disease. The study compares the effects of vitamin B complex (Vitamin B1 + B6 + B12) and ascorbic acid on angiogenesis using the Chorioallantoic Membrane (CAM) assay. The CAM of ten-day-old duck embryos were treated with various concentrations (1mg/ml, 3mg/ml, and 5mg/ml) of commercially available vitamin B complex and ascorbic acid, and the vascularization of the CAM was determined after two days of incubation. Results revealed that vitamin B complex and ascorbic acid have anti-angiogenic properties at peaks in dosages of 5mg/ml and 3mg/ml respectively. However, all concentrations of both vitamins have a significant effect on the growth of secondary blood vessels. The result of the post hoc test showed that vitamin B complex at a dosage of 5mg/ml exhibited the highest anti-angiogenic activity.

Keywords: angiogenesis, anti-angiogenic, property vitamin B complex, CAM assay

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